eng URI http://hdl.handle.net/10795/2954 βιολογία γενετική μικροοργανισμός κυτταρολογία Barrage formation has been traditionally used for the assessment of mycelial compatibility in many fungi and has often been assumed to represent a non-self recognition phenotype that is directly associated with vegetative incompatibility in these organisms. In this work, the optimal growth conditions for large-scale studies of barrage formation in the asexual fungus Verticillium dahliae were determined, and they were used for the analysis of a diverse collection comprising 69 isolates of V. dahliae and related species. Barrage formation was very frequent on a defined complete agar medium within V. dahliae and between species of the genus. However, it was not correlated with the classification of V. dahliae isolates into Vegetative Compatibility Groups (VCGs) (based on the standard method using complementary nit mutants), as it was recorded at high frequencies both within and between V. dahliae VCGs. The high overall frequency of barrage formation demonstrated the presence of a higher level of mycelial incompatibility in V. dahliae than heterokaryon incompatibility assessed by forcing complementary nit mutants to form heterokaryons under selective conditions. The possible association of barrage formation with morphological characteristics of the fungal colonies was investigated, and a negative correlation of frequency and intensity of barrages with the isolates’ capacity for pigment production was detected. Real-time quantitative PCR VCG discrimination 12 pp. LOMv1.0 creator LOMv1.0 publisher LOMv1.0 Scientific Coordinator LOMv1.0 Project Executing Organisation 2014-09-05 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Barrage formation Heterokaryon incompatibility Mycelial compatibility groups Mycelial interactions Non-selective compatibility tests Vegetative compatibility 545572 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2954/2/2954_1.96_%ce%94%ce%97%ce%9c_5_9_14.pdf URI http://hdl.handle.net/10795/2954 LOMv1.0creator 2016-04-27T08:14:15Z LOMv1.0validator 2016-04-27T08:14:15Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")