eng URI http://hdl.handle.net/10795/2706 ένζυμο βιοτεχνολογία ψυχανθές Plant glutathione transferases (GSTs) comprise a large family of inducible enzymes that play important roles in stress tolerance and herbicide detoxification. Treatment of Phaseolus vulgaris leaves with the aryloxyphenoxypropionic herbicide fluazifop-p-butyl resulted in induction of GST activities. Three inducible GST isoenzymes were identified and separated by affinity chromatography. Their full-length cDNAs with complete open reading frame were isolated using RACE-RT and information from N-terminal amino acid sequences. Analysis of the cDNA clones showed that the deduced amino acid sequences share high homology with GSTs that belong to phi and tau classes. The three isoenzymes were expressed in E. coli and their substrate specificity was determined towards 20 different substrates. The results showed that the fluazifop-inducible glutathione transferases from P. vulgaris (PvGSTs) catalyze a broad range of reactions and exhibit quite varied substrate specificity. Molecular modeling and structural analysis was used to identify key structural characteristics and to provide insights into the substrate specificity and the catalytic mechanism of these enzymes. These results provide new insights into catalytic and structural diversity of GSTs and the detoxifying mechanism used by P. vulgaris. 1 pp. LOMv1.0 creator LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2013-05 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Glutathione transferase Plant stress tolerance Herbicide detoxification Phaseolus vulgaris Common bean 892432 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2706/2/2706_Document-28.pdf 63rd Congress of the Hellenic Society of Biochemistry and Molecular Biology,9-11 Νοεμβρίου 2012, Κρήτη URI http://hdl.handle.net/10795/2706 LOMv1.0creator 2016-04-18T10:43:25Z LOMv1.0validator 2016-04-18T10:43:25Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")