eng URI http://hdl.handle.net/10795/2599 ασθένεια οφειλόμενη στη διατροφή μικροοργανισμός προϊόν διατροφής In the present study, the ability of Salmonella Typhimurium to form biofilm community on rocket leaves and rocket extract at 10 C and 20 C was investigated. This goal was achieved with the study of expression of genes associated with biofilm formation and other functional roles. The obtained results showed that Salmonella growth was inhibited when cultured in rocket extract (liquid and solid state) and when grew directly to rocket leaves. The observed inhibition might be attributed to nutrient starvation to the specific growth media because of plant leaves's variability, cell physiology and antimicrobial compounds of rocket. In addition, gene expression study using Real-Time PCR showed that biofilm was formatted on solid media, while the entrance and adhesion of the microorganism within the plant held more strongly through the stomata of the plant leaves. Furthermore, genes associated with managing stress situations were overexpressed at 20 C. From these results, it is indicated that further studies are needed to better determine the survival and/or growth of the pathogen as “real” biofilm cells on plants. In addition, the study on development and gene expression of biofilm cells is necessary in order to eliminate the specific pathogen and reduce the food-borne diseases it causes. 7 pp. LOMv1.0 creator LOMv1.0 publisher LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2015-08-08 Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Salmonella Rocket Biofilm formation Gene expression Stress response 406018 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2599/2/2599_Deliverable%20%28%ce%a020%20%ce%9441-44%29%20THALIS%20Biofilm%20MIS-380229%20Doulgeraki%20et%20al%20LWT%2065%2c254.pdf Περιοδικό: LWT - Food Science and Technology URI http://hdl.handle.net/10795/2599 LOMv1.0creator 2016-04-13T10:17:07Z LOMv1.0validator 2016-04-13T10:17:07Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")