eng URI http://hdl.handle.net/10795/2394 plant health product insecticide Baculovirus occlusion bodies, large proteinaceous structures which contain virions, have recently been engineered to incorporate foreign proteins. The major constituent protein of occlusion bodies from the baculovirus Autographa californica nucleopolyhedrovirus is polyhedrin, and assembly of recombinant occlusion bodies which incorporate a foreign protein depends on an interaction between native polyhedrin and a polyhedrin–foreign protein fusion. This technology has now been applied to the generation of a recombinant baculovirus (ColorBtrus) that produces occlusion bodies incorporating the Bacillus thuringiensis (Bt) insecticidal Cry1Ac toxin protein. ColorBtrus coexpresses native polyhedrin and a fusion protein in which polyhedrin is fused to the Bt toxin, which is in turn fused to green fluorescent protein (GFP). Analysis of ColorBtrus occlusion bodies confirmed that they include both Bt toxin and GFP, yet still incorporate virions. Bioassay of ColorBtrus demonstrated that its speed of action and pathogenicity are strikingly enhanced compared to wild-type virus. ColorBtrus represents a novel, powerful biological insecticide that combines positive attributes of both Bt toxin and baculovirus based systems. 9 pp. LOMv1.0 creator LOMv1.0 Project Final Beneficiary LOMv1.0 Project Executing Organisation 2003 LOMv1.0 training LOMv1.0 higher education 18+ Digital Library of the Operational Programme "Education and Lifelong Learning" abstract types Text Baculovirus Autographa californica nucleopolyhedrovirus AcMNPV Bacillus thuringiensis Bt Cry1Ac toxin Polyhedrin fusion proteins 326030 application/pdf http://repository.edulll.gr/edulll/bitstream/10795/2394/2/2394_GM%20Bacillus%20Thuringiensis.pdf Πανεπιστήμιο Θεσσαλίας. Σχολή Επιστημών Υγείας. Τμήμα Βιοχημείας και Βιοτεχνολογίας. Βιβλιογραφία διαλέξεων. Πρόγραμμα δια βίου μάθησης ΑΕΙ για την επικαιροποίηση γνώσεων αποφοίτων ΑΕΙ (ΠΕΓΑ) «Οι σύγχρονες τεχνικές βιο-ανάλυσης στην υγεία, τη γεωργία, το περιβάλλον και τη διατροφή» - Ενότητα 2. Σύγχρονες τεχνικές βιοανάλυσης στη γεωργία και το περιβάλλον URI http://hdl.handle.net/10795/2394 LOMv1.0creator 2016-03-18T07:54:00Z LOMv1.0validator 2016-03-18T07:54:00Z LOMv1.0 gre no no Copyright EYD-EPEDBM (Operational Programme "Education and Lifelong Learning")