The present paper aims at investigating Late Ro- man mosaic tesserae excavated in 2008 at the sanctuary of Isis and Sarapis, an archaeological site of ancient Messene situated in the south-western Peloponnese region of Greece. In order to further understand the production of tesserae during Roman times, both archaeological and scientific aspects will be considered, however, emphasis will be placed on the scientific analysis of the tesserae this in turn, leading to concrete findings which can further contribute to existing studies.