In the present study, the ability of S. Typhimurium to develop a biofilm community on rocket tissue was investigated at 20°C. The differences on expression of genes associated with several functional roles during growth of S. Typhimurium on rocket extract and rocket tissue regarding a laboratory growth medium (Luria – Bertani broth, LB) was also monitored. The findings of the present study could show that Salmonella reacts as exposed to different types of stress when inoculated to a heat sterile plant extract and plant tissue. However, further studies are needed to better determine the survival and / or growth of these as “real” biofilm cells on plant tissues.